Assessment of Physiological and Phytochemical Traits of Purple Coneflower (Echinacea purpurea L. Moench) in Response to Salicylic Acid and Methyl Jasmonate Application
Seyed Najmeddin Mortazavi1
1 Department of Horticultural Sciences, University of Zanjan, Zanjan, Iran
2 Department of Genetic Engineering and Plant Production, University of Zanjan, Zanjan, Iran
Medicinal Plant Research, 2021, Vol. 11, No. 1 doi: 10.5376/mpr.2021.11.0001
Received: 29 Oct., 2020 Accepted: 01 Feb., 2021 Published: 08 Feb., 2021
© 2021 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License
, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Mohebby M., Mortazavi S.N., Kheiry A., and Saba J., 2021, Assessment of physiological and phytochemical traits of purple coneflower (Echinacea purpurea L. Moench) in response to salicylic acid and methyl jasmonate application, Medicinal Plant Research, 11(1): 1-13 (doi: 10.5376/mpr.2021.11.0001)
Salicylic-acid and methyl-jasmonate are phytohormonal eilicitors that play important roles in regulating a number of physiological and phytochemical processes. For this purpose, a research was conducted as factorial based on complete randomized block design (CRBD) with three replications at the research field of agricultural faculty of University of Zanjan during farming years of 2017～2018 and 2018～2019. The first factor was salicylic-acid (0 (control), 50, 100 and 150 mM) and the second factor was methyl-jasmonate (0 (control), 50, 100 and 200 µM). In the first year of experiment, treatments were sprayed on plants (four plants per plot) in two stages (20 days apart). In the second year they were also sprayed on remaining plants as the first year. Results of ANOVA as factorial-split in time showed that at the level of one percent (p<0.01), the effect of salicylic-acid on catalase, peroxidase and superoxide dismutase enzymes traits and the effect of methyl-jasmonate on antioxidant activity and catalase enzyme traits and the interaction of salicylic-acid and methyl-jasmonate on antioxidant activity, catalase, peroxidase and superoxide dismutase enzymes traits were significant. Also, at the level of five percent (p<0.05), the effects of methyl-jasmonate on superoxide dismutase enzyme trait was significant. The interaction of salicylic-acid and methyl-jasmonate factor was also significant on total protein trait at the level of 10 percent (p<0.1). The highest amount of chlorogenic acid in the first year was in the treatment of 100 mM of salicylic-acid and 50 μM of methyl-jasmonate and the highest amount in the second year was in the treatment of 100 mM of salicylic-acid and 100 μM of methyl-jasmonate. Also, the highest amount of chicoric acid in the both years was in the treatment of 100 mM of salicylic-acid and 100 μM of methyl-jasmonate.
Antioxidant; Enzyme; Essential oils; Phenolic compounds; Phytohormone; Purple coneflower